Somatic and Flagellar Immunofluorescence of Salmonella

Identification of Salmonella somatic and flagellar antigens by modified serological methods.

This report describes two modified methods for the identification of Salmonella somatic (O) and flagellar (H) antigens. Over a period of 2 years, both modified methods were found to be approximately three times less labor intensive than the standard methods while requiring no more technical skill. The modified methods were as accurate as the standard methods in identifying the O and H antigens ...

Observations on Flagellar and Somatic Agglutination

Whole, shaken and heated suspensions of two Salmonella species were compared as to agglutinability, absorptive capacity and antigenic properties. The results were in general agreement with the flagellar antigen concept of Smith and Reagh. The removal of flagella by shaking or heating (100 degrees C.) resulted in altered agglutinability manifested by failure to give a floccular reaction with "wh...

Transduction of flagellar characters in Salmonella.

Wielofazowość antygenów rzęskowych u pałeczek Salmonella* Salmonella multiphasic flagellar antigen

U pałeczek Salmonella obserwuje się więcej niż jedną fazę antygenów rzęskowych, co jest uwarunkowane występowaniem jednego, dwóch lub trzech genów, które niezależnie od siebie kodują białko strukturalne witki rzęski o właściwościach antygenowych. Gen fliC koduje antygen rzęskowy pierwszej fazy, natomiast gen fljB – drugiej fazy. Trzecia faza antygenu rzęskowego związana jest z genem umiejscowio...

Flagellar hook protein from Salmonella SJ25.

From acid-disintegrated flagellar hooks of Salmonella SJ25 an immunochemically pure preparation of hook protein was obtained by column chromatography. The molecular weight of the protein determined by sodium dodecyl sulfate-gel electrophoresis was 43,000, whereas that of SJ25 flagellin was 56,000. The amino-terminal residue of the hook protein was determined to be seryl. The amino acid composit...